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resources:eval:user-guides:circuits-from-the-lab:eval-adpd410x:fluorescence [16 May 2022 02:14]
Joyce Velasco
resources:eval:user-guides:circuits-from-the-lab:eval-adpd410x:fluorescence [16 May 2022 02:32] (current)
Joyce Velasco
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 One method of measuring the amount of substance in a sample is by using fluorescent light. In this setup, a light is passed from a monochromatic source through the sample, and then the fluorescence in the substance is measured using a detector tuned to its wavelength. The intensity of the fluorescent light compared to the intensity of the incident light will be proportional to the amount of the fluorescent substance in the sample. An effective way of performing this is by using the setup shown below. ​ One method of measuring the amount of substance in a sample is by using fluorescent light. In this setup, a light is passed from a monochromatic source through the sample, and then the fluorescence in the substance is measured using a detector tuned to its wavelength. The intensity of the fluorescent light compared to the intensity of the incident light will be proportional to the amount of the fluorescent substance in the sample. An effective way of performing this is by using the setup shown below. ​
  
-Light is emitted from an LED at 365 nm wavelength. It then passes through a beam-splitter,​ which directs some of the incident light to a reference photodiode detector for sampling. Quinine in the sample fluoresces due to the 365 nm light and emits ~450 nm light. Another photodiode detector, sensitive to blue light frequencies,​ is positioned at 90 degrees from the light path to measure the intensity. This placement decreases the effects of light emitted from the source LED. Additionally,​ a monochromatic filter is placed in front of the detector to further isolate the measurement.\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​fluorescence_path.png?​nolink&​600|}}+Light is emitted from an LED at 365 nm wavelength. It then passes through a beam-splitter,​ which directs some of the incident light to a reference photodiode detector for sampling. Quinine in the sample fluoresces due to the 365 nm light and emits ~450 nm light. Another photodiode detector, sensitive to blue light frequencies,​ is positioned at 90 degrees from the light path to measure the intensity. This placement decreases the effects of the light emitted from the source LED. Additionally,​ a monochromatic filter is placed in front of the detector to further isolate the measurement.\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​fluorescence_path.png?​nolink&​600|}}
  
 ===== Demo Requirements ===== ===== Demo Requirements =====
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 ===== Setting up the EVAL-ADPD410X-ARDZ ===== ===== Setting up the EVAL-ADPD410X-ARDZ =====
-Configure the onboard jumper header and solder jumper connections as below\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​fl_adpd410x_jumperconn.jpg?​nolink&​400|}}\\ ​+Configure the onboard jumper header and solder jumper connectionsas shown below.\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​fl_adpd410x_jumperconn.jpg?​nolink&​400|}}\\ ​
  
 ^ **Header** ^ **Setting** ​              ^ **Image** ^ ^ **Header** ^ **Setting** ​              ^ **Image** ^
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 ===== Firmware Setup ===== ===== Firmware Setup =====
 Connect the EVAL-ADICUP3029 to the PC using the micro-USB to USB cable. Connect the EVAL-ADICUP3029 to the PC using the micro-USB to USB cable.
-Drag and drop the appropriate .hex file from the list below to the Daplink Drive (See [[:​resources:​eval:​user-guides:​circuits-from-the-lab:​eval-adpd410x#​driver-/​-firmware-setup]])+Drag and drop the appropriate .hex file from the list below to the Daplink Drive(See [[:​resources:​eval:​user-guides:​circuits-from-the-lab:​eval-adpd410x#​driver-/​-firmware-setup]])
  
 <WRAP todo> <WRAP todo>
 Prebuilt hex files can be found inside this {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​aducm3029_demo_adpd410x_waterquality.zip|zip archive}}. You can also find the latest build here: [[repo>​EVAL-ADICUP3029/​releases/​tag/​Latest]] Prebuilt hex files can be found inside this {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​aducm3029_demo_adpd410x_waterquality.zip|zip archive}}. You can also find the latest build here: [[repo>​EVAL-ADICUP3029/​releases/​tag/​Latest]]
-  * For EVAL-ADPD4100-ARDZADuCM3029_demo_adpd4100_waterquality.hex +  * For EVAL-ADPD4100-ARDZ, use ADuCM3029_demo_adpd4100_waterquality.hex 
-  * For EVAL-ADPD4100-ARDZADuCM3029_demo_adpd4101_waterquality.hex+  * For EVAL-ADPD4101-ARDZ, use ADuCM3029_demo_adpd4101_waterquality.hex
 </​WRAP>​ </​WRAP>​
  
 ===== Optical Path Setup ===== ===== Optical Path Setup =====
-The demo utilizes an optical path similar to the one used by [[ADI>​CN0503]] but only for a single channel. The single path base and cuvette holder are available as 3D-printable designs () and can also be ordered using Shapeways.\\ ​+The demo utilizes an optical path similar to the one used by [[ADI>​CN0503]]but only for a single channel. The single path base and cuvette holder are available as 3D-printable designs () and can also be ordered using Shapeways.\\ ​
   - Assemble the cuvette holder. See [[:​resources:​eval:​user-guides:​circuits-from-the-lab:​cn0503#​assembling_the_tower | Assembling the Tower]] for instructions.   - Assemble the cuvette holder. See [[:​resources:​eval:​user-guides:​circuits-from-the-lab:​cn0503#​assembling_the_tower | Assembling the Tower]] for instructions.
   - Insert the 365 nm LED Board to the base, as shown below.   - Insert the 365 nm LED Board to the base, as shown below.
   - Insert the Transmit Photodiode Board at the bottom of the base, as shown below. The Transmit Photodiode Board uses the same photodiode as the one used as reference in the [[ADI>​CN0503]].   - Insert the Transmit Photodiode Board at the bottom of the base, as shown below. The Transmit Photodiode Board uses the same photodiode as the one used as reference in the [[ADI>​CN0503]].
-  - Insert the Fluorescence Photodiode Board to the base as shown below. +  - Insert the Fluorescence Photodiode Board to the baseas shown below. 
-  - Insert the monochromatic or fluorescence filter to the slit in front of the Fluorescence Photodiode Board as show below.+  - Insert the monochromatic or fluorescence filter to the slit in front of the Fluorescence Photodiode Boardas shown below.
   - Insert the cuvette with the quinine sample to measure.   - Insert the cuvette with the quinine sample to measure.
  
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   - Connect the [[ADI>​EVAL-ADPD4100-ARDZ]] or [[ADI>​EVAL-ADPD4101-ARDZ]] to the [[ADI>​EVAL-ADICUP3029]].   - Connect the [[ADI>​EVAL-ADPD4100-ARDZ]] or [[ADI>​EVAL-ADPD4101-ARDZ]] to the [[ADI>​EVAL-ADICUP3029]].
   - Connect the EVAL-ADICUP3029 to the PC using the micro-USB cable and note the serial port from the Device Manager as in [[resources:​eval:​user-guides:​circuits-from-the-lab:​eval-adpd410x#​connection | Connection]].   - Connect the EVAL-ADICUP3029 to the PC using the micro-USB cable and note the serial port from the Device Manager as in [[resources:​eval:​user-guides:​circuits-from-the-lab:​eval-adpd410x#​connection | Connection]].
-  - Open command prompt or terminal and navigate ​to the examples folder inside the downloaded or cloned //​pyadi-iio//​ directory.+  - Open command prompt or terminal and navigate ​through ​the examples folder inside the downloaded or cloned //​pyadi-iio//​ directory.
   - Run the example script using the command. <​code>​...\pyadi-iio\examples>​python adpd410x_demo.py</​code>​   - Run the example script using the command. <​code>​...\pyadi-iio\examples>​python adpd410x_demo.py</​code>​
   - The script will ask for a serial port. Input the noted serial port and press Enter. In cases when the board is not found, press the reset button (S1) on the EVAL-ADPD4100-ARDZ or EVAL-ADPD4101-ARDZ and input the noted serial port again.\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​pyadiiio_example2_comport.png?​nolink&​400|}}   - The script will ask for a serial port. Input the noted serial port and press Enter. In cases when the board is not found, press the reset button (S1) on the EVAL-ADPD4100-ARDZ or EVAL-ADPD4101-ARDZ and input the noted serial port again.\\ {{:​resources:​eval:​user-guides:​circuits-from-the-lab:​adpd410x:​pyadiiio_example2_comport.png?​nolink&​400|}}
resources/eval/user-guides/circuits-from-the-lab/eval-adpd410x/fluorescence.txt · Last modified: 16 May 2022 02:32 by Joyce Velasco