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| resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate-measurement [20 Sep 2022 05:42] – draft 2 Michelle Claire So | resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate-measurement [20 Sep 2022 06:02] (current) – draft 3 Michelle Claire So | ||
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| - Click the Gear icon at the top right of the window to open Settings.\\ {{ : | - Click the Gear icon at the top right of the window to open Settings.\\ {{ : | ||
| - Select the correct COM Port of the device and connect (see {{ : | - Select the correct COM Port of the device and connect (see {{ : | ||
| - | - Load the configuration file for Nitrate Measurement {{ : | + | - Load the configuration file for Nitrate Measurement {{ : |
| - Configure the settings for path 3 with the desired name, set wavelength to 530 nm and select measurement type: absorption.\\ {{ : | - Configure the settings for path 3 with the desired name, set wavelength to 530 nm and select measurement type: absorption.\\ {{ : | ||
| - Add empty cuvette (or filled with distilled water) to the cuvette holder assembly and insert to path 3 and click Optimize LED. This properly sets the LED current in this path so that the light intensity is close to 50%.\\ {{ : | - Add empty cuvette (or filled with distilled water) to the cuvette holder assembly and insert to path 3 and click Optimize LED. This properly sets the LED current in this path so that the light intensity is close to 50%.\\ {{ : | ||
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| ==== Setting a Baseline Ratio ==== | ==== Setting a Baseline Ratio ==== | ||
| The baseline ratio is used to remove small factors introduced to the measurement by the optical glass elements, such as beam splitter, lens, and filters. The baseline ratio is the ARAT value of a known setups such as with an empty cuvette or distilled water sample where it is known that the ratio of incident and transmitted light should be approximately 1. This value is used as a reference for successive measurements which will be defined as the relative ratio RRAT. | The baseline ratio is used to remove small factors introduced to the measurement by the optical glass elements, such as beam splitter, lens, and filters. The baseline ratio is the ARAT value of a known setups such as with an empty cuvette or distilled water sample where it is known that the ratio of incident and transmitted light should be approximately 1. This value is used as a reference for successive measurements which will be defined as the relative ratio RRAT. | ||
| - | - Click OK on the settings window and on the main window select optical path 3 and display mode ARAT.\\ {{ : | + | - Click OK on the settings window and on the main window select optical path 3 and display mode ARAT.\\ {{ : |
| - | - Click on Start Measurement and note the average value on the graph. Remember that the solution should still be in the path. The average value will be used as the baseline ratio for this path. | + | - Click on Start Measurement and note the average value on the graph. Remember that the solution should still be in the path. The average value will be used as the baseline ratio for this path.\\ {{ : |
| - | - Record the average value and click on Stop Measurement then, click on the settings icon on the top right corner to bring back the settings and click on Advanced to bring up another window.\\ {{ : | + | - Record the average value and click on Stop Measurement then, click on the settings icon on the top right corner to bring back the settings and click on Advanced to bring up another window.\\ {{ : |
| - | - In the “Enter a direct command” text field, type “DEF1 RATB <average value> | + | - In the “Enter a direct command” text field, type “DEF1 RATB <average value> |
| - | - Optionally, you can write mg/L to the primary unit field of path 3. These are just labels and are not necessary.\\ {{ : | + | - Optionally, you can write mg/L to the primary unit field of path 3. These are just labels and are not necessary.\\ {{ : |
| - Click Okay here and on the settings window to go back to the main. Remove the empty cuvette/s or distilled water sample/s. The device is now ready to measure Nitrate. | - Click Okay here and on the settings window to go back to the main. Remove the empty cuvette/s or distilled water sample/s. The device is now ready to measure Nitrate. | ||
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| ==== Performing a Nitrate Measurement ==== | ==== Performing a Nitrate Measurement ==== | ||
| - | - Place a prepared cuvette with the test sample to path 3. Check the Preparing Test Sample section on how to prepare a cuvette with the test sample and indicator.\\ {{ : | + | - Place a prepared cuvette with the test sample to path 3. Check the Preparing Test Sample section on how to prepare a cuvette with the test sample and indicator.\\ {{ : |
| - Select path 3, set display mode to INS1, and press Start Measurement.\\ {{ : | - Select path 3, set display mode to INS1, and press Start Measurement.\\ {{ : | ||
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resources/eval/user-guides/circuits-from-the-lab/cn0503/nitrate-measurement.1663645339.txt.gz · Last modified: 20 Sep 2022 05:42 by Michelle Claire So