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resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate-measurement [19 Sep 2022 10:02] – draft 1 Michelle Claire Soresources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate-measurement [20 Sep 2022 06:02] (current) – draft 3 Michelle Claire So
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 The scientific method of colorimetry uses the Beer-Lambert law, which indicates that a solute's concentration is proportional to absorbance, to determine the concentration of colored compounds in solutions. Nitrate measurement with a coloring reagent is one use for colorimetry. The procedure for setting the EVAL-CN0503-ARDZ for nitrate measurement is described in this document. The scientific method of colorimetry uses the Beer-Lambert law, which indicates that a solute's concentration is proportional to absorbance, to determine the concentration of colored compounds in solutions. Nitrate measurement with a coloring reagent is one use for colorimetry. The procedure for setting the EVAL-CN0503-ARDZ for nitrate measurement is described in this document.
  
-===== General Description/Overview ===== +===== General Description ===== 
-The four-channel optical platform EVAL-CN0503-ARDZ can monitor fluorescence, absorbance, and scattering. The only measurements that can be made using the two middle channels/light streams are of absorbance and 180-degree scattering. In this demonstration, there is only one path used to evaluate absorbance for a single light wavelength.+The four-channel optical platform EVAL-CN0503-ARDZ can monitor fluorescence, absorbance, and scattering. The only measurements that can be made using the two middle channels/light streams are absorbance and 180-degree scattering. In this demonstration, there is only one path used to evaluate absorbance for a single light wavelength.
  
 A chemical reagent or indicator is required for measuring nitrate using colorimetry. Different indicators display varying color and light absorption patterns. The solution exhibits highly different color hues at the ends of the nitrate range because some indicators break down into various ions that have different characteristics. The Nitrate (NO3-) Test Kit, which includes two solutions, will be used for this demonstration. The mixture will turn yellow when Solution 1 is added, while Solution 2 includes test chemicals that produce all various color shades. Visual estimation or spectrophotometric measurement in the range of 4-40 mg/L at λmax 400 nm can be used to determine the nitrate concentration. As a result, the third path of the CN0503 will be illuminated by a light source with a wavelength of 530 nm in this demonstration. A chemical reagent or indicator is required for measuring nitrate using colorimetry. Different indicators display varying color and light absorption patterns. The solution exhibits highly different color hues at the ends of the nitrate range because some indicators break down into various ions that have different characteristics. The Nitrate (NO3-) Test Kit, which includes two solutions, will be used for this demonstration. The mixture will turn yellow when Solution 1 is added, while Solution 2 includes test chemicals that produce all various color shades. Visual estimation or spectrophotometric measurement in the range of 4-40 mg/L at λmax 400 nm can be used to determine the nitrate concentration. As a result, the third path of the CN0503 will be illuminated by a light source with a wavelength of 530 nm in this demonstration.
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 ===== Setting the EVAL-CN0503-ARDZ ===== ===== Setting the EVAL-CN0503-ARDZ =====
-Before starting with these steps, please check the [[resources:eval:user-guides:circuits-from-the-lab:cn0503|Hardware User Guide]] for the steps to assembling the CN0503. Additionally, please check the [[resources:eval:user-guides:eval-adicup3029:reference_designs:demo_cn0503|Software User Guide]] for the steps in setting up the firmware and the {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }} for running the software. This demo assumes that you already have an assembled board with a working firmware already programmed on the [[ADI>EVAL-ADICUP3029]] and a ready-to-run software in the host computer.+Before starting with these steps, please check the [[resources:eval:user-guides:circuits-from-the-lab:cn0503|Hardware User Guide]] for the steps to assemble the CN0503. Additionally, please check the [[resources:eval:user-guides:eval-adicup3029:reference_designs:demo_cn0503|Software User Guide]] for the steps in setting up the firmware and the {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }} for running the software. This demo assumes that you already have an assembled board with a working firmware already programmed on the [[ADI>EVAL-ADICUP3029]] and a ready-to-run software in the host computer.
  
 Configure the onboard jumper shunt connection as below: Configure the onboard jumper shunt connection as below:
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 | //LD2SEL and LD3SEL//    | Set to VARD | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ld2sel_-_ard.png?100 |}} {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ld3sel_-_ard.png?100 |                              }}                                               | | //LD2SEL and LD3SEL//    | Set to VARD | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ld2sel_-_ard.png?100 |}} {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ld3sel_-_ard.png?100 |                              }}                                               |
 | //IOSEL//                | Set to ARD  | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:iosel_-_ard.png?nolink&100 |}}                          | | //IOSEL//                | Set to ARD  | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:iosel_-_ard.png?nolink&100 |}}                          |
-| //P1.8V//                | Shorted     | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:p1.8v_-_ard.png?nolink&100 |}}     +| //P1.8V//                | Shorted     | {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:p1.8v_-_ard.png?nolink&100 |}}                          |
  
 ==== Initial Connection and Setup ==== ==== Initial Connection and Setup ====
-  - Connect the 530nm LED Board to LED3. \\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitarte_led_placement.png?nolink&600 |}}+  - Connect the 530nm LED Board to LED3. \\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_led_placement.png?nolink&600 |}}
   - Connect the [[ADI>EVAL-ADICUP3029]] to the [[ADI>CN0503]] and connect a microUSB-to-USB cable from the board to the host computer.   - Connect the [[ADI>EVAL-ADICUP3029]] to the [[ADI>CN0503]] and connect a microUSB-to-USB cable from the board to the host computer.
   - Run the software (using python scripts or the executable, see the {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }}) and wait for the main window to open.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ph_mainwindow.png?nolink&600 |}}   - Run the software (using python scripts or the executable, see the {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }}) and wait for the main window to open.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ph_mainwindow.png?nolink&600 |}}
-  - Click the Gear icon at the top right of the window to open Settings.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ph_opensettings.png?nolink&600 |}} +  - Click the Gear icon at the top right of the window to open Settings.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_open_settings.png?nolink&600 |}} \\ 
-  - Select the correct COM Port of the device and connect (see {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }} for help)\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ph_clickconnect.png?nolink&400 |}} +  - Select the correct COM Port of the device and connect (see {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_gui_quick_start_guide.docx | Quick Setup Guide }} for help)\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_click_connect.png?nolink&400 |}} 
-  - Load the configuration file for Nitrate Measurement {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_defaults_nitrate.zip |}}+  - Load the configuration file for Nitrate Measurement {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:cn0503_defaults_nitrate.zip |}} \\
   - Configure the settings for path 3 with the desired name, set wavelength to 530 nm and select measurement type: absorption.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_path_setting.png?nolink&200 |}}   - Configure the settings for path 3 with the desired name, set wavelength to 530 nm and select measurement type: absorption.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_path_setting.png?nolink&200 |}}
   - Add empty cuvette (or filled with distilled water) to the cuvette holder assembly and insert to path 3 and click Optimize LED. This properly sets the LED current in this path so that the light intensity is close to 50%.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_empty_cuvette.png?nolink&600 |}}   - Add empty cuvette (or filled with distilled water) to the cuvette holder assembly and insert to path 3 and click Optimize LED. This properly sets the LED current in this path so that the light intensity is close to 50%.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_empty_cuvette.png?nolink&600 |}}
 \\ \\
 ==== Setting a Baseline Ratio ==== ==== Setting a Baseline Ratio ====
-The baseline ratio is used to remove small factors introduced to the measurement such as by the optical glass elements, e.g., beam splitter, lens, and filters. The baseline ratio is the ARAT value of a known setups such as with an empty cuvette or distilled water sample where it is known that the ratio of incident and transmitted light should be approximately 1. This value is used as a reference for successive measurements which will be defined as the relative ratio RRAT. +The baseline ratio is used to remove small factors introduced to the measurement by the optical glass elements, such as beam splitter, lens, and filters. The baseline ratio is the ARAT value of a known setups such as with an empty cuvette or distilled water sample where it is known that the ratio of incident and transmitted light should be approximately 1. This value is used as a reference for successive measurements which will be defined as the relative ratio RRAT. 
-  - Click OK on the settings window and on the main window select optical path 3 and display mode ARAT.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitarte_arat.png?nolink&600 |}} +  - Click OK on the settings window and on the main window select optical path 3 and display mode ARAT.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitarte_arat1.png?nolink&600 |}} \\ 
-  - Click on Start Measurement and note the average value on the graph. Remember that the solution should still be in the path. The average value will be used as the baseline ratio for this path.  +  - Click on Start Measurement and note the average value on the graph. Remember that the solution should still be in the path. The average value will be used as the baseline ratio for this path.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_average_value.png?nolink&600 |}} \\ 
-  - Record the average value and click on Stop Measurement then, click on the settings icon on the top right corner to bring back the settings and click on Advanced to bring up another window.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitarte_stop-and-advance.png?nolink&600 |}} +  - Record the average value and click on Stop Measurement then, click on the settings icon on the top right corner to bring back the settings and click on Advanced to bring up another window.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitarte_stop-and-advance.png?nolink&600 |}} \\ 
-  - In the “Enter a direct command” text field, type “DEF1 RATB <average value>” for path 3, then press Send Command. Replace the <average value> to the value you have noted earlier. +  - In the “Enter a direct command” text field, type “DEF1 RATB <average value>” for path 3, then press Send Command. Replace the <average value> to the value you have noted earlier and click ok.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_ratb.png?nolink&600 |}}\\ 
-  - Optionally, you can write mg/L to the primary unit field of path 3. These are just labels and are not necessary.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_unit.png?nolink&600 |}}+  - Optionally, you can write mg/L to the primary unit field of path 3. These are just labels and are not necessary.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_unit.png?nolink&600 |}} \\
   - Click Okay here and on the settings window to go back to the main. Remove the empty cuvette/s or distilled water sample/s. The device is now ready to measure Nitrate.   - Click Okay here and on the settings window to go back to the main. Remove the empty cuvette/s or distilled water sample/s. The device is now ready to measure Nitrate.
 \\ \\
  
 ==== Performing a Nitrate Measurement ==== ==== Performing a Nitrate Measurement ====
-  - Place a prepared cuvette with the test sample to path 3. Check the Preparing Test Sample section on how to prepare a cuvette with the test sample and indicator.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_sample.png?nolink&600 |}}+  - Place a prepared cuvette with the test sample to path 3. Check the Preparing Test Sample section on how to prepare a cuvette with the test sample and indicator.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_sample.png?nolink&600 |}} \\
   - Select path 3, set display mode to INS1, and press Start Measurement.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_curve.png?nolink&600 |}}   - Select path 3, set display mode to INS1, and press Start Measurement.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_curve.png?nolink&600 |}}
 \\ \\
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   - Create a scatter plot of the two columns with RRAT as the X-variable and Nitrate level as the y-Variable\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_curve.png?nolink&600 |}} \\   - Create a scatter plot of the two columns with RRAT as the X-variable and Nitrate level as the y-Variable\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_curve.png?nolink&600 |}} \\
   - Create a trend-line for the scatter plot and select the polynomial option with an order of up to 5 depending on characteristic of the curve. Also, check the box at the bottom to Display Equation on chart.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_trendline_options.png?nolink&400 |}} \\   - Create a trend-line for the scatter plot and select the polynomial option with an order of up to 5 depending on characteristic of the curve. Also, check the box at the bottom to Display Equation on chart.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_trendline_options.png?nolink&400 |}} \\
-  - Use the equation of the trendline and input the coefficients to the CN0503 using the Command field in the Advanced settings. See the [[resources:eval:user-guides:eval-adicup3029:reference_designs:demo_cn0503|Software User Guide]] for details on the command.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:ph_polycommand.png?nolink&600 |}}+  - Use the equation of the trendline and input the coefficients to the CN0503 using the Command field in the Advanced settings. See the [[resources:eval:user-guides:eval-adicup3029:reference_designs:demo_cn0503|Software User Guide]] for details on the command.\\ {{ :resources:eval:user-guides:circuits-from-the-lab:cn0503:nitrate_direct_command.png?nolink&600 |}}
 \\ \\
 ===== Reference Links ===== ===== Reference Links =====
resources/eval/user-guides/circuits-from-the-lab/cn0503/nitrate-measurement.txt · Last modified: 20 Sep 2022 06:02 by Michelle Claire So

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