This version is outdated by a newer approved version.
This version (11 May 2021 18:15) is a draft.
Approvals: 0/1
This version (11 May 2021 18:15) is a draft.Approvals: 0/1
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Table of Contents
Optical Platform Fluorescence Measurement (EVAL-CN0503-ARDZ)
Fluorescence occurs when the electrons of certain chemical compounds are excited by beam of light causing them to emit light at a different and typically longer wavelength. The intensity of the emitted fluorescent light is linear for a broad range of concentration of the substance. The method of measuring fluorescence intensity to obtain the concentration of the material is advantageous over standard colorimetry due to its specificity and resistance to noise. Since only the target chemical compound emits light at a different wavelength, using a detector or optical filter which detects or passes narrowband light centered at that wavelength decreases interference from the source or incident light.
General Description/Overview
The EVAL-CN0503-ARDZ is a four-channel optical platform capable of fluorescence, absorbance and scattering measurements for liquid solutions. Getting the concentration of a compound in a liquid solution using fluorescence involves either directly measuring the emitted light from the compound or from an added a reagent which introduced fluorescent material proportional to the concentration of the target compound. The sensor used is the fluorescence photodiode placed perpendicular to the light path from the source LED. This photodiode is sensitive to longer wavelength light than the transmit photodiode placed directly in the light path. Its position and spectral sensitivity help to remove any interference from the source LED to the fluorescent light samples. Additionally, an optical fluorescent filter (narrowband longpass filter) is inserted in the slot in front of the sensor to further isolate fluorescent light from noise.
The demo shows how directly measuring fluorescent light from tonic water indicates the presence and level of quinine.
Demo Requirements
The following is a list of items needed in order to replicate this demo.
- EVAL-CN0503-ARDZ, completely assembled (see Hardware User Guide)
- EVAL-ADICUP3029 with firmware (see Software User Guide)
- Host computer with CN0503 software (see Quick Setup Guide) and Microsoft Excel (Optional for generating your own measurement curve)
- Tonic water from commercial brands
Setting the EVAL-CN0503-ARDZ
Before starting with these steps, please check the Hardware User Guide for the steps to assembling the EVAL-CN0503-ARDZ. Additionally, please check the Software User Guide for the steps in setting up the firmware and the Quick Setup Guide for running the software. This demo assumes that you already have an assembled board with a working firmware already programmed on the EVAL-ADICUP3029 and a ready-to-run software in the host computer.
Configure the onboard jumper shunt connection as below:
| Jumper Header | Setting |
|---|---|
| LD2SEL and LD3SEL | Set to VARD |
| IOSEL | Set to ARD |
| P1.8V | Shorted |
resources/eval/user-guides/circuits-from-the-lab/cn0503/fluorescence.1620749753.txt.gz · Last modified: 11 May 2021 18:15 by Angelo Nikko Catapang