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| resources:eval:user-guides:circuits-from-the-lab:cn0503:colorimetry [13 Jul 2021 14:14] – Angelo Nikko Catapang | resources:eval:user-guides:circuits-from-the-lab:cn0503:colorimetry [21 Jul 2021 08:48] (current) – Angelo Nikko Catapang | ||
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| - | ====== Optical Platform | + | ====== Optical Platform: pH Measurement Demo ====== |
| - | An important water quality indicator for almost any use is the presence | + | Colorimetry can be used to determine |
| ===== General Description/ | ===== General Description/ | ||
| - | The EVAL-CN0503-ARDZ is a four-channel optical platform capable of fluorescence, | + | The EVAL-CN0503-ARDZ is a four-channel optical platform capable of fluorescence, |
| Measuring pH using colorimetry involves using a chemical reagent or indicator. Different indicators exhibit different color and light absorbance behavior. Some indicators break down into different ions which also exhibit different properties which is why the solution has very different color hues at the ends of the pH range. For this demo we will be using bromothymol blue from either a prepared solution or the API pH Test Kit. Bromothymol blue separates into a weak acid (HIn) and a conjugate base (In). HIn has high absorbance to 430nm light while In has high absorbance at 615nm light. The demo will use light sources with these two wavelengths for the two middle channels.\\ | Measuring pH using colorimetry involves using a chemical reagent or indicator. Different indicators exhibit different color and light absorbance behavior. Some indicators break down into different ions which also exhibit different properties which is why the solution has very different color hues at the ends of the pH range. For this demo we will be using bromothymol blue from either a prepared solution or the API pH Test Kit. Bromothymol blue separates into a weak acid (HIn) and a conjugate base (In). HIn has high absorbance to 430nm light while In has high absorbance at 615nm light. The demo will use light sources with these two wavelengths for the two middle channels.\\ | ||
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| ^ Jumper Header | ^ Jumper Header | ||
| - | | //LD2SEL and LD3SEL// | + | | //LD2SEL and LD3SEL// |
| - | | // | + | | // |
| - | | // | + | | // |
| ==== Initial Connection and Setup ==== | ==== Initial Connection and Setup ==== | ||
resources/eval/user-guides/circuits-from-the-lab/cn0503/colorimetry.1626178466.txt.gz · Last modified: 13 Jul 2021 14:14 by Angelo Nikko Catapang